Fig. 5

Characterization and secretion changes of microglia-derived exosomes. a Flow chart of exosome isolation. b Morphology image of exosomes acquired by TEM. Scale bar = 100 nm. c Western blot image showing the expression of exosome markers (CD63 and CD81) and inflammatory markers (IL-1β and P2X7) in LPS-stimulated exosomes. d–i Analysis of the particle number and size of exosomes from the different treatment groups by a Flow Nano Analyzer